Production and action of interferon in mice infected with rabies virus
Identifieur interne : 000461 ( France/Analysis ); précédent : 000460; suivant : 000462Production and action of interferon in mice infected with rabies virus
Auteurs : R. Marcovistz [France] ; H. Siang [France] ; A. G. Hovanessian [France]Source :
- Annales de l'Institut Pasteur / Virologie [ 0769-2617 ] ; 1984.
Descripteurs français
- Wicri :
- topic : Rage.
English descriptors
- KwdEn :
Abstract
Summary: This study describes the production and action of interferon in mice infected with rabies virus (challenge virus strain). The production of interferon was monitored by an in vitro assay of plasma and extract of spleen, lung and brain for interferon activity. The action of interferon in mice was assessed by measuring two interferon-mediated enzyme activities, 2–5A synthetase and poly(I)poly(C)-Sepharose-bound protein kinase. Mice (strain C3H/He) infected with rabies virus were sacrificed at different time intervals, and the level of both enzyme activities was measured in extracts of spleen, lung and brain. Inoculation of mice with a mouse brain homogenate containing rabies virus resulted in the production of interferon with two different peaks detectable in the plasma: an early peak 24–48 h after inoculation followed by another peak on the sixth day of infection. The first peak was found to be independent of rabies virus, since it was also detectable in control mice injected with a brain homogenate from a normal uninfected mouse. The second peak of interferon, however, was probably due to the presence of high levels of interferon in the brain in response to rabies virus replication. In accord with the production of interferon, the level of 2–5A synthetase and protein kinase was enhanced several-fold in the different organs of mice infected with rabies virus. The level of protein kinase activity in the brain was strikingly higher than that of the spleen or the lung of mice 4 days after infection, and this level remained elevated until the death of the animals. These results indicate that infection of mice with rabies virus results in the production of interferon which is active in the infected mice, since different organs respond to it by elevated levels of 2–5A synthetase and protein kinase. Interferon produced during rabies virus infection, however, seems to be ineffective in inhibiting replication of virus, since these mice do not survive.
Url:
DOI: 10.1016/S0769-2617(84)80037-4
Affiliations:
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ISTEX:996F9B43294E05B639D9F75028E3C3C8EB6C46DBLe document en format XML
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The production of interferon was monitored by an in vitro assay of plasma and extract of spleen, lung and brain for interferon activity. The action of interferon in mice was assessed by measuring two interferon-mediated enzyme activities, 2–5A synthetase and poly(I)poly(C)-Sepharose-bound protein kinase. Mice (strain C3H/He) infected with rabies virus were sacrificed at different time intervals, and the level of both enzyme activities was measured in extracts of spleen, lung and brain. Inoculation of mice with a mouse brain homogenate containing rabies virus resulted in the production of interferon with two different peaks detectable in the plasma: an early peak 24–48 h after inoculation followed by another peak on the sixth day of infection. The first peak was found to be independent of rabies virus, since it was also detectable in control mice injected with a brain homogenate from a normal uninfected mouse. The second peak of interferon, however, was probably due to the presence of high levels of interferon in the brain in response to rabies virus replication. In accord with the production of interferon, the level of 2–5A synthetase and protein kinase was enhanced several-fold in the different organs of mice infected with rabies virus. The level of protein kinase activity in the brain was strikingly higher than that of the spleen or the lung of mice 4 days after infection, and this level remained elevated until the death of the animals. These results indicate that infection of mice with rabies virus results in the production of interferon which is active in the infected mice, since different organs respond to it by elevated levels of 2–5A synthetase and protein kinase. Interferon produced during rabies virus infection, however, seems to be ineffective in inhibiting replication of virus, since these mice do not survive.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Marcovistz, R" sort="Marcovistz, R" uniqKey="Marcovistz R" first="R." last="Marcovistz">R. Marcovistz</name></region><name sortKey="Hovanessian, A G" sort="Hovanessian, A G" uniqKey="Hovanessian A" first="A. G." last="Hovanessian">A. G. Hovanessian</name><name sortKey="Siang, H" sort="Siang, H" uniqKey="Siang H" first="H." last="Siang">H. Siang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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